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c igf 1r phosphorylation levels  (R&D Systems)


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    R&D Systems c igf 1r phosphorylation levels
    C Igf 1r Phosphorylation Levels, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 7—Baf60 deficiency enhances the expression and secretion of IGF2. A: Heat map representation of Baf60-regulated genes encoding secreted factors. B: qPCR analysis of gene expression in quadriceps muscle from AKO (n = 3), CKO (n = 6), ACKO (n = 4–5) mice and their littermate controls (Ctrl) on standard chow diet. C: IGF2 secretion in ex vivo cultured soleus (left) or plantaris (right) muscles. D: Plasma IGF2 concentration in chow-fed control and ACKO mice (n = 4–5). E: qPCR analysis of quadriceps gene expression in HFD-fed mice (left, n = 5–11) and plasma IGF2 concentrations in HFD-fed mice (right, n = 7–9). F: qPCR analysis of quadriceps gene expression (left) and plasma IGF2 levels (right) in old control and ACKO mice (n = 6–7). G: ChIP assays on control and ACKO muscle nuclei using antibodies against histone 3 acetylation (AcH3) and H3K9 demethylation (H3K9me2). Location of primers on the proximal regions of three IGF2 promoters is shown. H: Schematic model demonstrating the regulation of exercise preformation and systemic energy homeostasis by Baf60a and Baf60c. Data in B–G represent mean 6 SEM. *P , 0.05, **P , 0.01, ***P , 0.001.

    Journal: Diabetes

    Article Title: Uncoupling Exercise Bioenergetics From Systemic Metabolic Homeostasis by Conditional Inactivation of Baf60 in Skeletal Muscle.

    doi: 10.2337/db17-0367

    Figure Lengend Snippet: Figure 7—Baf60 deficiency enhances the expression and secretion of IGF2. A: Heat map representation of Baf60-regulated genes encoding secreted factors. B: qPCR analysis of gene expression in quadriceps muscle from AKO (n = 3), CKO (n = 6), ACKO (n = 4–5) mice and their littermate controls (Ctrl) on standard chow diet. C: IGF2 secretion in ex vivo cultured soleus (left) or plantaris (right) muscles. D: Plasma IGF2 concentration in chow-fed control and ACKO mice (n = 4–5). E: qPCR analysis of quadriceps gene expression in HFD-fed mice (left, n = 5–11) and plasma IGF2 concentrations in HFD-fed mice (right, n = 7–9). F: qPCR analysis of quadriceps gene expression (left) and plasma IGF2 levels (right) in old control and ACKO mice (n = 6–7). G: ChIP assays on control and ACKO muscle nuclei using antibodies against histone 3 acetylation (AcH3) and H3K9 demethylation (H3K9me2). Location of primers on the proximal regions of three IGF2 promoters is shown. H: Schematic model demonstrating the regulation of exercise preformation and systemic energy homeostasis by Baf60a and Baf60c. Data in B–G represent mean 6 SEM. *P , 0.05, **P , 0.01, ***P , 0.001.

    Article Snippet: Then, 200 mL reagent diluent was added to each sample to dissolve, from which 100 mL was used to measure insulin-like growth factor 2 (IGF2) levels using a Mouse IGF-II DuoSet ELISA Kit (R&D systems) according to the manufacturer’s protocol.

    Techniques: Expressing, Gene Expression, Ex Vivo, Cell Culture, Muscles, Clinical Proteomics, Concentration Assay, Control

    IGFs and related protein levels in the plasma and CSF

    Journal: Journal of Neuroinflammation

    Article Title: Insulin-like growth factors and related proteins in plasma and cerebrospinal fluids of HIV-positive individuals

    doi: 10.1186/s12974-015-0288-6

    Figure Lengend Snippet: IGFs and related protein levels in the plasma and CSF

    Article Snippet: IGF2 levels were also quantified by ELISA (ALPCO Diagnostics, NH: cat # 22-IGF-E30, sensitivity 20 pg/ml) using the core service of the Einstein Diabetes Research Center (Biomarker Analytic Research Core, Bronx, NY, USA).

    Techniques: Clinical Proteomics

    Comparison of IGFs and related proteins in plasma and CSF in subjects with or without neurocognitive impairment

    Journal: Journal of Neuroinflammation

    Article Title: Insulin-like growth factors and related proteins in plasma and cerebrospinal fluids of HIV-positive individuals

    doi: 10.1186/s12974-015-0288-6

    Figure Lengend Snippet: Comparison of IGFs and related proteins in plasma and CSF in subjects with or without neurocognitive impairment

    Article Snippet: IGF2 levels were also quantified by ELISA (ALPCO Diagnostics, NH: cat # 22-IGF-E30, sensitivity 20 pg/ml) using the core service of the Einstein Diabetes Research Center (Biomarker Analytic Research Core, Bronx, NY, USA).

    Techniques: Comparison, Clinical Proteomics